glosensor kit Search Results


90
Promega non-lytic glosensor cyclic amp assay kit
(A) Luminescent reporter calcium assay showing typical responses to varying doses (5 nM to 10 µM) of DA. (B) Dose–response curves of the CHO cells transfected with the D1 and aequorin constructs and treated with DA and norepinephrine. Antagonistic effect of butaclamol on the D1 receptor. (C) Luminescent responses to DA (3.3 µM) in the CHO cells expressing D1 receptor and aequorin. The cells were pretreated with 5 or 30 µM butaclamol (red and black lines, respectively) for 15 minutes. (D) Percent responses to DA (3.3 µM) when the cells were pretreated with different concentrations of butaclamol. Note that only 30 µM butaclamol was effective in blocking the DA action on the D1 receptor. (E) Luminescent reporter cAMP assay <t>(GloSensor)</t> showing typical responses to varying doses (0.1 pM to 1 µM) of DA. (B) Dose–response curves of the HEK cells transfected with the D1 and GloSensor constructs for DA and norepinephrine. Bars indicate standard errors for a minimum of four replicated plates.
Non Lytic Glosensor Cyclic Amp Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+kit/pmc03031531-166-7-13?v=Promega
Average 90 stars, based on 1 article reviews
non-lytic glosensor cyclic amp assay kit - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

90
Promega glosensor kit
(A) Luminescent reporter calcium assay showing typical responses to varying doses (5 nM to 10 µM) of DA. (B) Dose–response curves of the CHO cells transfected with the D1 and aequorin constructs and treated with DA and norepinephrine. Antagonistic effect of butaclamol on the D1 receptor. (C) Luminescent responses to DA (3.3 µM) in the CHO cells expressing D1 receptor and aequorin. The cells were pretreated with 5 or 30 µM butaclamol (red and black lines, respectively) for 15 minutes. (D) Percent responses to DA (3.3 µM) when the cells were pretreated with different concentrations of butaclamol. Note that only 30 µM butaclamol was effective in blocking the DA action on the D1 receptor. (E) Luminescent reporter cAMP assay <t>(GloSensor)</t> showing typical responses to varying doses (0.1 pM to 1 µM) of DA. (B) Dose–response curves of the HEK cells transfected with the D1 and GloSensor constructs for DA and norepinephrine. Bars indicate standard errors for a minimum of four replicated plates.
Glosensor Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/glosensor+kit/us09658208-108-22-37?v=Promega
Average 90 stars, based on 1 article reviews
glosensor kit - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


(A) Luminescent reporter calcium assay showing typical responses to varying doses (5 nM to 10 µM) of DA. (B) Dose–response curves of the CHO cells transfected with the D1 and aequorin constructs and treated with DA and norepinephrine. Antagonistic effect of butaclamol on the D1 receptor. (C) Luminescent responses to DA (3.3 µM) in the CHO cells expressing D1 receptor and aequorin. The cells were pretreated with 5 or 30 µM butaclamol (red and black lines, respectively) for 15 minutes. (D) Percent responses to DA (3.3 µM) when the cells were pretreated with different concentrations of butaclamol. Note that only 30 µM butaclamol was effective in blocking the DA action on the D1 receptor. (E) Luminescent reporter cAMP assay (GloSensor) showing typical responses to varying doses (0.1 pM to 1 µM) of DA. (B) Dose–response curves of the HEK cells transfected with the D1 and GloSensor constructs for DA and norepinephrine. Bars indicate standard errors for a minimum of four replicated plates.

Journal: PLoS ONE

Article Title: Evidence for D1 Dopamine Receptor Activation by a Paracrine Signal of Dopamine in Tick Salivary Glands

doi: 10.1371/journal.pone.0016158

Figure Lengend Snippet: (A) Luminescent reporter calcium assay showing typical responses to varying doses (5 nM to 10 µM) of DA. (B) Dose–response curves of the CHO cells transfected with the D1 and aequorin constructs and treated with DA and norepinephrine. Antagonistic effect of butaclamol on the D1 receptor. (C) Luminescent responses to DA (3.3 µM) in the CHO cells expressing D1 receptor and aequorin. The cells were pretreated with 5 or 30 µM butaclamol (red and black lines, respectively) for 15 minutes. (D) Percent responses to DA (3.3 µM) when the cells were pretreated with different concentrations of butaclamol. Note that only 30 µM butaclamol was effective in blocking the DA action on the D1 receptor. (E) Luminescent reporter cAMP assay (GloSensor) showing typical responses to varying doses (0.1 pM to 1 µM) of DA. (B) Dose–response curves of the HEK cells transfected with the D1 and GloSensor constructs for DA and norepinephrine. Bars indicate standard errors for a minimum of four replicated plates.

Article Snippet: For cAMP response assays, we used the non-lytic GloSensor cyclic AMP assay kit (Promega) for monitoring intracellular cAMP in live cells.

Techniques: Calcium Assay, Transfection, Construct, Expressing, Blocking Assay, cAMP Assay